Vimentin Rabbit Monoclonal Antibody(ARA732)
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
Product Details
WB | 1:5000-1:10000 |
IHC | 1:100-1:200 |
IF/ICC | 1:2000-1:10000 |
FC | 1:10-1:200 |
IP | 1:20 |
Description | Rabbit Monoclonal Antibody to Vimentin |
Antibody Type | Primary antibody |
Predicted MW | 54kDa |
Immunogen | A synthetic peptide corresponding to residues on the C-terminus of human Vimentin was used as an immunogen. |
Purification | ProA affinity purified IgG |
Form/Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.07%. |
Alternative Names | Vimentin |
Gene Symbol | VIM |
Entrez Gene | 7431(Human) |
Swissprot | P08670 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
All lanes: Anti-Vimentin antibody at 1:5,000 dilution
Predicted MW: 54 kDa
Observed MW: 54 kDa
Lane 1: Hela
Lane 2: HEK293
Lane 3: A549
Lane 4: 3T3
Lysate at 10 μg per lane
2nd Ab:
G&R HRP(H+L) 1:10,000
Exposure: 100s
All lanes: Anti-Vimentin antibody at 1:5,000 dilution
Predicted MW: 54 kDa
Observed MW: 54 kDa
Lane 1: Mu Heart
Lane 2: Rat Heart
Lysate at 10 μg per lane
2nd Ab:
G&R HRP(H+L) 1:10,000
Exposure: 20s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling vimentin with ARA732 at 1:100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Overlay histogram showing Hela cells stained with ARA732 (Blue). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (ARA732, 1:10 dilution) in 1x PBS/1% BSA for 30 min at 4°C. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1:2,000 dilution for 20 min at 4°C. Unlabelled sample (Red) was used as a control.
ARA732 staining Vimentin in Hela cells by IF/ICC (immunofluorescence/Immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:2,000) at 4°C. An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (1:500).
Vimentin was immunoprecipitated from 0.4mg of Hela lysate with ARA732 at 1:20 dilution.
2nd Ab:
GAR HRP for IP 1:500
Lane 1: ARA732 IP in Hela whole cell lysate
Lane 2: Rabbit IgG instead of ARA732 in Hela whole cell lysate
Lane3: Hela whole cell lysate, 10 μg (input)
Exposure: 60s
FAQs
New Products
