VCP Mouse Monoclonal Antibody(C3825)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
Product Details
WB | 1:500 - 1:2000 |
IHC | 1:50 - 1:200 |
IF/ICC | 1:10 - 1:50 |
FC | 1:10 - 1:50 |
Description | Mouse monoclonal antibody to VCP |
Specificity | Recognizes endogenous levels of VCP protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human VCP. The exact sequence is proprietary. |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 89 kD; Observed: 95 kD |
Form/Buffer | Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Transitional endoplasmic reticulum ATPase; TER ATPase; 15S Mg(2+)-ATPase p97 subunit; Valosin-containing protein; VCP |
Gene Symbol | VCP |
Entrez Gene | 7415(Human); 269523(Mouse); 116643(Rat) |
SwissProt | P55072(Human); Q01853(Mouse); P46462(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of VCP expression in Hela (A), K562 (B), A549 (C), C6 (D), U87 MG (E), NIH3T3 (F) whole cell lysates. (Predicted band size: 89 kD; Observed band size: 95 kD)
Immunohistochemical analysis of VCP staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of VCP staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of K562 cells using Anti-VCP Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AREX® Fluor 488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
FAQs
New Products
