ULK4 Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:2000 |
IHC | 1:50 - 1:200 |
IF/ICC | 1:50 - 1:200 |
Description | Rabbit polyclonal antibody to ULK4 |
Specificity | Recognizes endogenous levels of ULK4 protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human ULK4 |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 142 kD; Observed: 160 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Serine/threonine-protein kinase ULK4; Unc-51-like kinase 4 |
Gene Symbol | ULK4 |
Entrez Gene | 54986(Human); 209012(Mouse) |
SwissProt | Q96C45(Human); Q3V129(Mouse) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of ULK4 expression in mouse brain (A), rat lung (B) whole cell lysates. (Predicted band size: 142 kD; Observed band size: 160 kD)
Immunohistochemical analysis of ULK4 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ULK4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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