TMEM25 Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:2000 |
IF/ICC | 1:50 - 1:200 |
Description | Rabbit polyclonal antibody to TMEM25 |
Specificity | Recognizes endogenous levels of TMEM25 protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human TMEM25. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 23; Observed: 50 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Transmembrane protein 25 |
Gene Symbol | TMEM25 |
Entrez Gene | 84866(Human); 71687(Mouse) |
SwissProt | Q86YD3(Human); Q9DCF1(Mouse) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of TMEM25 expression in OVCAR3 (A), mouse kidney (B), rat brain (C) whole cell lysates. (Predicted band size: 23; 33; 34; 37; 39 kD; Observed band size: 50 kD)
Immunofluorescent analysis of TMEM25 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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