TFIIF RAP 30 Rabbit Monoclonal Antibody(C874)
MSDS 
Key features and details
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Product Details
WB | 1:500 - 1:1000 |
IHC | 1:50 - 1:200 |
IF/ICC | 1:50 - 1:200 |
Description | Recombinant rabbit monoclonal antibody to TFIIF RAP 30 |
Specificity | Recognizes endogenous levels of TFIIF RAP 30 protein |
Antibody Type | Primary antibody, Recombinant |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within human TFIIF RAP 30. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 28 kD; Observed: 28 kD |
Form/Buffer | Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. |
Alternative Names | RAP30; General transcription factor IIF subunit 2; ATP-dependent helicase GTF2F2; General transcription factor IIF 30 kDa subunit; Transcription initiation factor IIF subunit beta; TFIIF-beta; Transcription initiation factor RAP30 |
Gene Symbol | GTF2F2 |
Entrez Gene | 2963(Human) |
SwissProt | P13984(Human) |
Western blot analysis of TFIIF RAP 30 expression in A549 (A), Hela (B), K562 (C) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 28 kD)
Immunohistochemical analysis of TFIIF RAP 30 staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of TFIIF RAP 30 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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