SGPP1 kanin polyklonal antikropp

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Nyckelfunktioner och detaljer

Kanin polyklonal antikropp mot SGPP1

Mål: SGPP1
Källa/värd: Kanin
Reaktivitet: Människan
Klonalitet: Polyklonal
Applikationer: WB, IHC
Konjugering: Okonjugerad
Lagring: vid-20°C

Märke:

KAT.NR. : APA17944

US$ Välj

Storlek:

50 μL 100 μL

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Produktinformation

Bakgrund

Specifically dephosphorylates sphingosine 1-phosphate (S1P), dihydro-S1P, and phyto-S1P. Does not act on ceramide 1-phosphate, lysophosphatidic acid or phosphatidic acid , dihydro-S1P, and phyto-S1P. Does not act on ceramide 1-phosphate, lysophosphatidic acid or phosphatidic acid . Sphingosine-1-phosphate phosphatase activity is needed for efficient recycling of sphingosine into the sphingolipid synthesis pathway . Regulates the intracellular levels of the bioactive sphingolipid metabolite S1P that regulates diverse biological processes acting both as an extracellular receptor ligand or as an intracellular second messenger . Involved in efficient ceramide synthesis from exogenous sphingoid bases. Converts S1P to sphingosine, which is readily metabolized to ceramide via ceramide synthase.

Ansökan

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Översikt

Beskrivning	Kanin polyklonal antikropp mot SGPP1
Specificitet	Känner igen endogena nivåer av SGPP1-protein.
Antikroppstyp	Primär antikropp
Imnunogen	KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human SGPP1. The exact sequence is proprietary.
Rening	The antibody was purified by immunogen affinity chromatography.
Molekylvikt	Förutspått: 49 kD; Observerad: 49 kD
Form/buffert	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternativa namn	Sphingosine-1-phosphate phosphatase 1; SPPase1; Spp1; hSPP1; hSPPase1; Sphingosine-1-phosphatase 1
Gensymbol	SGPP1
Entrez Gene	81537(Människa)
SwissProt	Q9BX95(Human)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of SGPP1 expression in human placenta (A) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 49 kD)

Immunohistochemical analysis of SGPP1 staining in human placenta formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Förvaring

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Obs

For Research Use Only. Not for use in diagnostic procedures.

Vanliga frågor

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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