IL-23 alpha Rabbit Monoclonal Antibody(C1428)
MSDS
Key features and details
Recombinant rabbit monoclonal antibody to IL-23 alpha
- Mål:
- Källa/värd:
- Reaktivitet:
- Klonalitet:
- Applikationer:
- Konjugering:
- Lagring:
-
Märke:
Produktinformation
Bakgrund
Associates with IL12B to form the pro-inflammatory cytokine IL-23 that plays different roles in innate and adaptive immunity . Released by antigen-presenting cells such as dendritic cells or macrophages, binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R to activate JAK2 and TYK2 which then phosphorylate the receptor to form a docking site leading to the phosphorylation of STAT3 and STAT4 . This process leads to activation of several pathways including p38 MAPK or NF-kappa-B and promotes the production of pro-inflammatory cytokines such as interleukin-17A/IL17A . In turn, participates in the early and effective intracellular bacterial clearance .
Ansökan
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
Översikt
Beskrivning | Recombinant rabbit monoclonal antibody to IL-23 alpha |
Specificitet | Recognizes endogenous levels of IL-23 alpha protein |
Antikroppstyp | Primary antibody, Recombinant |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within human IL-23 alpha protein. The exact sequence is proprietary. |
Rening | The antibody was purified by immunogen affinity chromatography. |
Molekylvikt | Predicted: 20 kD; Observed: 21 kD |
Form/buffert | Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. |
Alternativa namn | SGRF; Interleukin-23 subunit alpha; IL-23 subunit alpha; IL-23-A; Interleukin-23 subunit p19; IL-23p19 |
Gensymbol | IL23A |
Entrez Gene | 51561 (människa) |
SwissProt | Q9NPF7(Människa) |
Data
Western blot analysis of IL-23 alpha expression in K562 (A), PC3 (B) whole cell lysates. (Predicted band size: 20 kD; Observed band size: 21 kD)
Förvaring
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Obs
For Research Use Only. Not for use in diagnostic procedures.
Vanliga frågor
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
Nya produkter
