Humant IFN beta ARExSet® antikroppspar för ELISA

Interleukin 2 (IL-2), även känd som T-cellstillväxtfaktor (TCGF), är en 15-18 kDa variabelt glykosylerad alfa -helixpolypeptid som är en medlem av den gemensamma gammakedjan (gamma c) cytokinfamiljen. Den existerar som en monomer och har en anmärkningsvärt kort halveringstid (< 30 minutes). Human IL-2 is synthesized as a 153 amino acid (aa) precursor that contains a 20 aa signal sequence plus a 133 aa mature region. The mature region is alpha -helical in nature, and contains one utilized O-linked glycosylation site at Thr3 plus three cysteines, two of which form an intrachain disulfide bond that is essential for activity. Mature human IL-2 shares 73%, 66%, 78% and 97% aa identity with canine, rat, feline and rhesus monkey IL-2, respectively. Although human IL-2 shares only approximately 60% aa identity with the highly polymorphic mouse IL-2, human IL-2 is known to be active on mouse IL-2 responsive cells. Cells reported to secrete IL-2 include gamma δ T cells, activated conventional CD4+ and CD8+ T cells, neurons, microglia, and hematopoietic stem cells.The receptor for IL-2 (IL-2 R) is composed of three subunits, the 55 kDa CD25/IL-2 R alpha chain, the 70 kDa IL-2 R beta chain, and the 65 kDa Common gamma Chain. IL-2 first binds to CD25, the binary complex then recruits IL-2 R beta and gamma c to form the quaternary signaling complex. In addition to IL-2, IL-2 R beta is used by IL-15 in its quaternary signaling complex. gamma c also serves as a signaling receptor for IL-4, -7, -9, -15, and -21.In vitro studies have shown an important role for IL-2 in T cell activation and expansion. In vivo, IL-2 is critical for the development, maintenance and function of regulatory T cells (Treg) which provide protection against autoimmune disease. On the other hand, IL-2 can also promote autoimmune inflammation in target organs through its roles in regulating the expression of T cell trafficking genes, and production of Th2 cytokines. Within the CD8+ T cell subset, IL-2 is essential for optimal primary responses and differentiation into terminal effector cells. IL-2 also promotes the development of activated CD8+ T cells into memory cells.

pg/ml	O.D.		Genomsnittlig	Rättad
0.00	0.0153	0.0164	0.0159
15.63	0.0908	0.0819	0.0864	0.0705
31.25	0.1504	0.1490	0.1497	0.1339
62.50	0.2953	0.2956	0.2955	0.2796
125.00	0.5887	0.5429	0.5658	0.5500
250.00	1.1280	1.0380	1.0830	1.0672
500.00	1.9580	1.7880	1.8730	1.8572
1000.00	3.1140	3.1170	3.1155	3.0997

• Optimerade infångnings- och detektionsantikroppsparningar med rekommenderade koncentrationer sparar lång utvecklingstid
• Utvecklingsprotokoll tillhandahålls för att vägleda ytterligare analysoptimering
• Analysen kan anpassas efter dina specifika behov
• Ekonomiskt alternativ till kompletta kit

• Fånga antikroppar
• Detektionsantikropp
• Rekombinant standard
• Streptavidin konjugerat till pepparrot-peroxidas (Streptavidin-HRP)

ARExSet^®Tilläggsreagenssats (5 plattor): innehåller mikroplattor med 96 brunnar, plattförseglare, substratlösning, stopplösning, plattbeläggningsbuffert (PBS), tvättbuffert och analysbuffert.

Mikroplattor med 96 brunnar: AREX Biosciences, Katalog # DSEP01. 

Plattförseglare: AREX Biosciences, Katalog # DSSF01.

Beläggningsbuffert:137 mM NaCl, 2,7 mM KCl, 8,1 mM Na₂HPO₄1,5 mM KH₂PO₄, pH 7,2-7,4, 0,2 μm filtrerad. AREX Biosciences, Katalog # DSCB01.

Blockerande buffert: AREX Biosciences, Katalog # DSBB01.

Tvättbuffert: 0,05 % Tween 20 i PBS, pH 7,2-7,4. AREX Biosciences, Katalog # DSWB01.

Analysbuffert: 0,5%BSA, 0,05%Tween20, PBS Solution.AREX Biosciences, Katalog # DSAB01

Substratlösning:Tetrametylbensidin. AREX Biosciences, Katalog # DSTS01.

Stoppa lösning: 0,5 mol/ml H₂SO₄. AREX Biosciences, Katalog # DSSS01.