Human Asprosin ELISA Kit
KAT.NR. : AEH0032
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Bakgrund
Asprosin is a new type of adipocytokine discovered by Romere et al. in 2016. It is mainly produced and secreted by white adipocytes, which can bind to the receptors on the surface of hepatocytes on the one hand, stimulate the glucose output of hepatocytes, and directly increase blood sugar; On the other hand, it can penetrate the blood-brain barrier, activate the neuronal activity of agRP gene-associated protein (AgRP) associated with feeding-related in the hypothalamic center, and inhibit the neuronal activity of anorexia-related opioid-melanocyte corticogen (POMC), promote food intake, and indirectly exert a hypoglycemic effect.
Typiska data

| ng/ml | O.D. | Genomsnittlig | Rättad | |
| 0.00 | 0.0407 | 0.0468 | 0.0438 | |
| 4.69 | 0.1037 | 0.1032 | 0.1035 | 0.0597 |
| 9.38 | 0.1406 | 0.1638 | 0.1522 | 0.1085 |
| 18.75 | 0.2698 | 0.2895 | 0.2797 | 0.2359 |
| 37.50 | 0.5256 | 0.5766 | 0.5511 | 0.5074 |
| 75.00 | 1.0950 | 1.1600 | 1.1275 | 1.0838 |
| 150.00 | 2.3230 | 2.0280 | 2.1755 | 2.1318 |
| 300.00 | 3.6020 | 3.3780 | 3.4900 | 3.4463 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Provnummer | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Medel(ng/ml) | 6.4 | 29.6 | 112.8 | 5.9 | 31.2 | 118.1 |
| Standardavvikelse | 0.3 | 1.2 | 5.9 | 0.3 | 1.3 | 5.4 |
| Variationskoefficient (%) | 4 | 4 | 5.2 | 5.1 | 4.2 | 4.6 |
Intra-assayprecision (precision inom en analys) Tre prover med känd koncentration testades tjugo gånger på en platta för att bedöma intra-assayprecisionen.
Inter-assayprecision (precision mellan analyser) Tre prover med känd koncentration testades sex gånger på en platta för att bedöma intra-assayprecisionen.
Exempelvärden
| Provmatris | Prov utvärderat | Område (ng/ml) | Detekterbar (%) | Medelvärde för detekterbar (ng/ml) |
| Serum | 30 | 3,35-128,74 | 100 | 34.32 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Asprosin in this assay. Ingen medicinsk historia var tillgänglig för donatorerna.
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