Histon H3 (MonoMethyl-K79) Monoklonal musantikropp (C2149)

MSDS

Histone H3 (MonoMethyl-K79) Mouse Monoclonal Antibody(C2149)

Nyckelfunktioner och detaljer

Mus monoklonal antikropp mot Histone H3 (MonoMethyl-K79)

Mål: Histon H3 (MonoMethyl-K79)
Källa/värd: Mus
Reaktivitet: Människa, mus, råtta
Klonalitet: Monoklonal
Applikationer: WB, IHC
Konjugering: Okonjugerad
Lagring: vid-20°C

Märke:

KAT.NR. : AMA01761

US$ Välj

Storlek:

50 μL 100 μL

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Produktdetaljer

Bakgrund

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Ansökan

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:1000 - 1:3000
IHC	1:200 - 1:500

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Översikt

Beskrivning	Mus monoklonal antikropp mot Histone H3 (MonoMethyl-K79)
Specificitet	Känner igen endogena nivåer av Histone H3-protein endast när Mono-metyleras vid K79.
Antikroppstyp	Primär antikropp
Imnunogen	KLH-conjugated synthetic Mono-methylated peptide corresponding to residues surrounding K79 of human Histone H3 protein. The exact sequence is proprietary.
Rening	Antikroppen renades genom immunogenaffinitetskromatografi.
Molekylvikt	Förutspått: 15 kD; Observerad: 17 kD
Form/buffert	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternativa namn	HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3
Gensymbol	HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST2H3A; HIST2H3C; HIST2H3D; H3F3A; H3F3B
Entrez Gene	8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968(Human); 319152; 15077; 15078(Mouse); 291159; 100361558(Rat)
SwissProt	P68431; Q71DI3; P84243(Human); P68433; P84228; P84244(mus); Q6LED0; P84245(råtta)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of Histone H3 (MonoMethyl-K79) expression in Hela (A), Raw264.7 (B), rat testis (C) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)

Immunohistochemical analysis of Histone H3 (MonoMethyl-K79) staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Förvaring

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Obs

Endast för forskningsanvändning. Ej för användning i diagnostiska procedurer.

Vanliga frågor

Vilka är huvudtyperna av forskningsantikroppar och hur skiljer de sig åt?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

Hur vet jag om en forskningsantikropp är lämplig för mitt experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Kan felaktig förvaring av forskningsantikroppar påverka experimentella resultat?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Varför fungerar inte den rekommenderade utspädningen i databladet bra i mitt experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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