CREB (Phospho-S129) kanin polyklonal antikropp

MSDS

CREB (Phospho-S129) Rabbit Polyclonal Antibody

Nyckelfunktioner och detaljer

Kanin polyklonal antikropp mot CREB (Phospho-S129)

Mål: CREB (Phospho-S129)
Källa/värd: Kanin
Reaktivitet: Människan, mus, råtta, nötkreatur, kyckling, gris, får, zebrafisk
Klonalitet: Polyklonal
Applikationer: WB, IHC, IF/ICC
Konjugering: Okonjugerad
Lagring: vid-20°C

Märke:

KAT.NR. : APA10562

US$ Välj

Storlek:

50 μL 100 μL

Trail, bulkstorlek eller anpassade önskemål Vänligen kontakta oss

Produktinformation

Bakgrund

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters . Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation , a sequence present in many viral and cellular promoters . Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation . Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells . Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling .

Ansökan

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:2000
IHC	1:50 - 1:200
IF/ICC	1:50 - 1:100

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Översikt

Beskrivning	Kanin polyklonal antikropp mot CREB (Phospho-S129)
Specificitet	Känner igen endogena nivåer av CREB-protein endast när det fosforyleras vid S129.
Antikroppstyp	Primär antikropp
Imnunogen	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S129 of human CREB protein. The exact sequence is proprietary.
Rening	Antikroppen renades genom immunogenaffinitetskromatografi.
Molekylvikt	Förutspått: 35 kD; Observerad: 38 kD
Form/buffert	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternativa namn	Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1
Gensymbol	CREB1
Entrez Gene	1385 (Human); 12912(mus); 81646(råtta)
SwissProt	P16220 (Human); Q01147(mus); P15337(råtta)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of CREB (Phospho-S129) expression in RAW264.7 UV-treated (A) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 38 kD)

Immunohistochemical analysis of CREB (Phospho-S129) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of CREB (Phospho-S129) staining in RAW264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Förvaring

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Obs

Endast för forskningsanvändning. Ej för användning i diagnostiska procedurer.

Vanliga frågor

Vilka är huvudtyperna av forskningsantikroppar och hur skiljer de sig åt?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

Hur vet jag om en forskningsantikropp är lämplig för mitt experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Kan felaktig förvaring av forskningsantikroppar påverka experimentella resultat?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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