BCAS4 Rabbit Polyclonal Antibody

MSDS

Key features and details

Rabbit polyclonal antibody to BCAS4

Mål: BCAS4
Källa/värd: Kanin
Reaktivitet: Människan
Klonalitet: Polyklonal
Applikationer: WB, IHC
Konjugering: Okonjugerad
Lagring: vid-20°C

Märke:

KAT.NR. : APA10391

US$ Välj

Storlek:

50 μL 100 μL

Trail, Bulk size or Custom requests Please contact us

Produktinformation

Bakgrund

Breast carcinoma-amplified sequence 4 (BCAS4). Belongs to the cappuccino family Subcellular location: Cytoplasm Tissue specificity: Brain, thymus, spleen, kidney and placenta. Overexpressed in most breast cancer cell lines

Ansökan

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:100 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Översikt

Beskrivning	Rabbit polyclonal antibody to BCAS4
Specificitet	Recognizes endogenous levels of BCAS4 protein.
Antikroppstyp	Primär antikropp
Imnunogen	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BCAS4. The exact sequence is proprietary.
Rening	The antibody was purified by immunogen affinity chromatography.
Molekylvikt	Predicted: 22 kD; Observed: 23 kD
Form/buffert	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternativa namn	Breast carcinoma-amplified sequence 4
Gensymbol	BCAS4
Entrez Gene	55653(Människa)
SwissProt	Q8TDM0(människa)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of BCAS4 expression in A431 (A), K562 (B) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 23 kD)

Immunohistochemical analysis of BCAS4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Förvaring

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Obs

For Research Use Only. Not for use in diagnostic procedures.

Vanliga frågor

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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