ALPL kanin monoklonal antikropp (C3118)

MSDS

Nyckelfunktioner och detaljer

Kanin monoklonal antikropp mot ALPL

Mål: ALPL
Källa/värd: Kanin
Reaktivitet: Människa, mus, råtta
Klonalitet: Monoklonal
Applikationer: WB, IHC, IF/ICC, IP
Konjugering: Okonjugerad
Lagring: vid-20°C

Märke:

KAT.NR. : AMA02730

US$ Välj

Storlek:

50 μL 100 μL

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Produktdetaljer

Bakgrund

Alkaline phosphatase that metabolizes various phosphate compounds and plays a key role in skeletal mineralization and adaptive thermogenesis . Has broad substrate specificity and can hydrolyze a considerable variety of compounds: however, only a few substrates, such as diphosphate (inorganic pyrophosphate; PPi), pyridoxal 5'-phosphate (PLP) and N-phosphocreatine are natural substrates . Plays an essential role in skeletal and dental mineralization via its ability to hydrolyze extracellular diphosphate, a potent mineralization inhibitor, to phosphate: it thereby promotes hydroxyapatite crystal formation and increases inorganic phosphate concentration .

Ansökan

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:50 - 1:100
IF/ICC	1:50 - 1:100
IP	1:10 - 1:50

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Översikt

Beskrivning	Kanin monoklonal antikropp mot ALPL
Specificitet	Känner igen endogena nivåer av ALPL-protein.
Antikroppstyp	Primär antikropp
Imnunogen	A synthetic peptide of human Alkaline Phosphatase, Tissue Non-Specific
Rening	Antikroppen renades genom immunogenaffinitetskromatografi.
Molekylvikt	Förutspått: 57 kD; Observerad: 70-80 kD
Form/buffert	Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Alternativa namn	Alkaline phosphatase tissue-nonspecific isozyme; AP-TNAP; TNSALP; Alkaline phosphatase liver/bone/kidney isozyme
Gensymbol	ALPL
Entrez Gene	249(Människa); 11647(mus); 25586(råtta)
SwissProt	P05186 (Human); P09242(mus); P08289(råtta)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of ALPL expression in Hela (A), C6 (B) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 70-80 kD)

Immunohistochemical analysis of ALPL staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of ALPL staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.

Förvaring

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Obs

Endast för forskningsanvändning. Ej för användning i diagnostiska procedurer.

Vanliga frågor

Vilka är huvudtyperna av forskningsantikroppar och hur skiljer de sig åt?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

Hur vet jag om en forskningsantikropp är lämplig för mitt experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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