STRAD alpha Rabbit Polyclonal Antibody
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:100 |
FC | 1:10 - 1:30 |
Description | Rabbit polyclonal antibody to STRAD alpha |
Specificity | Recognizes endogenous levels of STRAD alpha protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human STRAD alpha |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 33; Observed: 48 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | LYK5; STRAD; STE20-related kinase adapter protein alpha; STRAD alpha; STE20-related adapter protein; Serologically defined breast cancer antigen NY-BR-96 |
Gene Symbol | STRADA |
Entrez Gene | 92335(Human) |
SwissProt | Q7RTN6(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of STRAD alpha expression in Jurkat (A) whole cell lysates. (Predicted band size: 33; 34; 38; 41; 43; 48 kD; Observed band size: 48 kD)
Immunofluorescent analysis of STRAD alpha staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of A549 cells using Anti-STRAD alpha Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AREX® Fluor 488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
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