SMAD3 Rabbit Monoclonal Antibody(C917)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IHC | 1:50 - 1:200 |
IF/ICC | 1:50 - 1:200 |
Description | Recombinant rabbit monoclonal antibody to SMAD3 |
Specificity | Recognizes endogenous levels of SMAD3 protein |
Antibody Type | Primary antibody, Recombinant |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within human SMAD3. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 48 kD; Observed: 50 kD |
Form/Buffer | Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. |
Alternative Names | MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3 |
Gene Symbol | SMAD3 |
Entrez Gene | 4088(Human); 17127(Mouse); 25631(Rat) |
SwissProt | P84022(Human); Q8BUN5(Mouse); P84025(Rat) |
Western blot analysis of SMAD3 expression in A549 (A), THP1 (B), mouse kidney (C), rat kidney (D), rat muscle (E) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 50 kD)
Immunohistochemical analysis of SMAD3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AREX® Fluor 488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).
Immunofluorescent analysis of SMAD3 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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