Siglec 15 Mouse Monoclonal Antibody(C2959)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IHC | 1:100 - 1:500 |
IF/ICC | 1:100 - 1:500 |
FC | 1:100 - 1:200 |
Description | Mouse monoclonal to Siglec 15 |
Specificity | Recognizes endogenous levels of Siglec 15 protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human Siglec 15 expressed in HEK293 |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 36 kD; Observed: 60 kD kD |
Form/Buffer | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | CD33L3; SialIC, acid-binding Ig-like lectin 15; Siglec-15; CD33 antigen-like 3 |
Gene Symbol | SIGLEC15 |
Entrez Gene | 284266(Human) |
SwissProt | Q6ZMC9(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of Siglec 15 expression in PC2 (A), LNCap (B), HEK293 (C), PC3 (D), DU145 (E), COS7 (F), HEK293-6e (G) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 60 kD kD)
Immunohistochemical analysis of Siglec 15 staining in human cervical cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Siglec 15 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of Jurkat cells using Anti-Siglec 15 Antibody (green) and negative control (red).
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