RYK Mouse Monoclonal Antibody(C3768)
WB | 1:500 - 1:1000 |
FC | 1:10 - 1:50 |
Description | Mouse monoclonal antibody to RYK |
Specificity | Recognizes endogenous levels of RYK protein. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RYK. The exact sequence is proprietary. |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 67 kD; Observed: 68 kD |
Form/Buffer | Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | JTK5A; Tyrosine-protein kinase RYK |
Gene Symbol | RYK |
Entrez Gene | 6259(Human) |
SwissProt | P34925(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Western blot analysis of RYK expression in human lung (A) whole cell lysates. (Predicted band size: 67 kD; Observed band size: 68 kD)

Flow cytometric analysis of A431 cells using Anti-RYK Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AREX® Fluor 488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
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