RSK3 Rabbit Monoclonal Antibody(C3217)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IHC | 1:50 - 1:100 |
IF/ICC | 1:50 - 1:100 |
IP | 1:10 - 1:50 |
Description | Rabbit monoclonal antibody to RSK3 |
Specificity | Recognizes endogenous levels of RSK3 protein. |
Antibody Type | Primary antibody |
Imnunogen | A synthetic peptide of human RSK3 |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 83 kD; Observed: 90 kD |
Form/Buffer | Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. |
Alternative Names | MAPKAPK1C; RSK3; Ribosomal protein S6 kinase alpha-2; S6K-alpha-2; 90 kDa ribosomal protein S6 kinase 2; p90-RSK 2; p90RSK2; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c; Ribosomal S6 kinase 3; RSK-3; pp90RSK3 |
Gene Symbol | RPS6KA2 |
Entrez Gene | 6196(Human) |
SwissProt | Q15349(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of RSK3 expression in CHOK1 (A), Hela (B) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 90 kD)
Immunohistochemical analysis of RSK3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of RSK3 staining in U87MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.
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