RB1 Mouse Monoclonal Antibody(C2938)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:100 - 1:500 |
FC | 1:100 - 1:200 |
Description | Mouse monoclonal to RB1 |
Specificity | Recognizes endogenous levels of RB1 protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human RB1 expressed in E. Coli |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 106 kD; Observed: 110 kD kD |
Form/Buffer | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110 |
Gene Symbol | RB1 |
Entrez Gene | 5925(Human) |
SwissProt | P06400(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of RB1 expression in Jurkat (A), A431 (B) whole cell lysates. (Predicted band size: 106 kD; Observed band size: 110 kD kD)
Immunofluorescent analysis of RB1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of Jurkat cells using Anti-RB1 Antibody (green) and negative control (red).
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