RABGGTB Rabbit Polyclonal Antibody

MSDS

Key features and details

Rabbit polyclonal antibody to RABGGTB

Target: RABGGTB
Source/Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Polyclonal
Applications: WB, IHC
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : APA16656

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Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of Rab proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX, such as RAB1A, RAB3A, RAB5A and RAB7A . Catalytic subunit of the geranylgeranyl transferase type 3 (GGTase-3) complex . The GGTase-3 complex geranylgeranylates and targets FBXL2 to the cellular membranes, where FBXL2 forms part of the E3 ubiquitin-protein ligase complex SCF(FBXL2) that mediates the degradation of membrane-anchored proteins . The GGTase-3 complex geranylgeranylates Golgi v-SNARE protein YKT6 at 'Cys-194' and this prenylation is required for Golgi SNARE complex assembly .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:2000
IHC	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit polyclonal antibody to RABGGTB
Specificity	Recognizes endogenous levels of RABGGTB protein
Antibody Type	Primary antibody
Imnunogen	Recombinant fusion protein of human RABGGTB. The exact sequence is proprietary.
Purification	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 36 kD; Observed: 37 kD
Form/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	GGTB; Geranylgeranyl transferase type-2 subunit beta; Geranylgeranyl transferase type II subunit beta; GGTase-II-beta; Rab geranyl-geranyltransferase subunit beta; Rab GG transferase beta; Rab GGTase beta; Rab geranylgeranyltransferase subunit beta; Type II protein geranyl-geranyltransferase subunit beta
Gene Symbol	RABGGTB
Entrez Gene	5876(Human); 19352(Mouse); 25533(Rat)
SwissProt	P53611(Human); P53612(Mouse); Q08603(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of RABGGTB expression in HeLa (A), HepG2 (B), mouse kidney (C), mouse liver (D), rat brain (E) whole cell lysates. (Predicted band size: 36 kD; Observed band size: 37 kD)

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for use in diagnostic procedures.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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