RAB9A Rabbit Monoclonal Antibody(C879)
MSDS 
Key features and details
Recombinant rabbit monoclonal antibody to RAB9A
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
CAT.NO. : AMA00491
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Product Details
Background
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion . RAB9A is involved in the transport of proteins between the endosomes and the trans-Golgi network (TGN) . Specifically uses NDE1/NDEL1 as an effector to interact with the dynein motor complex in order to control retrograde trafficking of RAB9-associated late endosomes to the TGN .
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
Overview
Description | Recombinant rabbit monoclonal antibody to RAB9A |
Specificity | Recognizes endogenous levels of RAB9A protein |
Antibody Type | Primary antibody, Recombinant |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within human RAB9A. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 22 kD; Observed: 23 kD |
Form/Buffer | Liquid in PBS, pH 7.4, containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. |
Alternative Names | RAB9; Ras-related protein Rab-9A |
Gene Symbol | RAB9A |
Entrez Gene | 9367(Human); 56382(Mouse); 84589(Rat) |
SwissProt | P51151(Human); Q9R0M6(Mouse); Q99P75(Rat) |
Data
Western blot analysis of RAB9A expression in A549 (A), Hela (B), K562 (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 23 kD)
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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