pTau217 Mouse Monoclonal Antibody(ARA957)

Datasheet

MSDS

pTau217 Mouse Monoclonal Antibody(ARA957)

Key features and details

Target: pTau217
Host: Mouse
Reactivity: Human
Clonality: Monoclonal
Application: Sandwich ELISA
Storage: -20°C

Brand:

CAT.NO. : ARA6806

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Size:

100μL

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Product Details

Background

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
Sandwich ELISA	1:250 - 1:500

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Species Reactivity	Human
Clonality	Monoclonal
Isotype	Mouse / IgG1
Immunogen	Synthetic peptide within aa200-250 of Tau. The exact peptide sequence is proprietary information
Cross Reactivity	Not tested
Recombinant	Not recombinant
Purification	Protein A purification
Form	Liquid
Conjugation	Unconjugated

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blotting analysis of pTau217 by ARA957 Synthetic pTau peptide fragments containing phosphorylated threonine 217 were crosslinked with BSA (phospho T217). The conjugates were run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. Peptide with phosphorylated threonine 231 or threonine 181 or without any phosphorylation site were processed with the same procedure as negative controls. ARA957 was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Tau (phospho T217) band was visualized using ECL Western Blotting Substrate.- Lane 1: 25 ng of Tau (phospho T217)-BSA conjugate- Lane 2: 25 ng of Tau (phospho T231)-BSA conjugate- Lane 3: 25 ng of Tau (phospho T181)-BSA conjugate- Lane 4: 25 ng of Tau Result: ARA957 can specifically detect Tau (phospho T217) by Western blotting.

Sandwich ELISA analysis of pTau217

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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