Prohibitin Rabbit Monoclonal Antibody(ARA999)
Key features and details
- Reactivity:
- Application:
- Host:
- Clonality:
- Target:
-
Brand:
CAT.NO. : ARA6904
US$ Please choose
US$ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.
Application
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:1000 |
|
IHC |
1:50 - 1:100 |
|
IF/ICC |
1:50 - 1:100 |
|
IP |
1:10 - 1:50 |
Overview
|
Product Description |
Rabbit monoclonal antibody to Prohibitin |
|
Immunogen |
A synthetic peptide of human Prohibitin |
|
Purification Method |
The antibody was purified by immunogen affinity chromatography. |
|
Clonality |
Monoclonal |
|
Product Form |
Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. |
|
Gene Name |
PHB1 |
|
Alternative Name |
Prohibitin |
|
Human Gene ID |
5245 |
|
Rat Gene ID |
25344 |
|
Human Protein ID |
P35232 |
|
Rat Protein ID |
P67779 |
Data
Western blot analysis of Prohibitin expression in Hela (A), A549 (B), HL60 (C), U2OS (D), C6 (E) whole cell lysates. (Predicted band size: 30 kD; Observed band size: 30 kD)
Immunohistochemical analysis of Prohibitin staining in human cholangiocarcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Prohibitin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
New Products
