PLA2G1B Rabbit Polyclonal Antibody

Datasheet

MSDS

Key features and details

Target: PLA2G1B
Host: Rabbit
Reactivity: Human, Mouse
Clonality: Polyclonal
Application: Sandwich ELISA
Storage: -20°C

Brand:

CAT.NO. : ARA6891

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Phospholipase A2 catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:500 - 1:1000
IHC	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Product Description	Rabbit polyclonal antibody to PLA2G1B
Immunogen	Recombinant fusion protein of human PLA2G1B. The exact sequence is proprietary.
Purification	The antibody was purified by immunogen affinity chromatography.
Clonality	Polyclonal
Form/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Gene Name	PLA2G1B
Alternative Names	PLA2; PLA2A; PPLA2; Phospholipase A2; Group IB phospholipase A2; Phosphatidylcholine 2-acylhydrolase 1B
Human Gene ID	5319
Mouse Gene ID	18778
Human Protein ID	P04054
Mouse Protein ID	Q3ZOY2

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of PLA2G1B expression in A549 (A), mouse pancreas (B) whole cell lysates. (Predicted band size: 16 kD; Observed band size: 15 kD)

Immunohistochemical analysis of PLA2G1B staining in mouse pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Component

Product Description	Rabbit polyclonal antibody to PLA2G1B
Immunogen	Recombinant fusion protein of human PLA2G1B. The exact sequence is proprietary.
Purification Method	The antibody was purified by immunogen affinity chromatography.
Clonality	Polyclonal
Product Form	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Gene Name	PLA2G1B
Alternative Names	PLA2; PLA2A; PPLA2; Phospholipase A2; Group IB phospholipase A2; Phosphatidylcholine 2-acylhydrolase 1B
Human Gene ID	5319
Mouse Gene ID	18778
Human Protein ID	P04054
Mouse Protein ID	Q3ZOY2

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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