PINK1 Mouse Monoclonal Antibody(C3989)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:1000 - 1:2000 |
IHC | 1:50 - 1:200 |
IF/ICC | 1:50 - 1:100 |
Description | Mouse monoclonal antibody to PINK1 |
Specificity | Recognizes endogenous levels of PINK1 protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human PINK1. The exact sequence is proprietary. |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 62 kD; Observed: 62 kD |
Form/Buffer | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Serine/threonine-protein kinase PINK1, mitochondrial; BRPK; PTEN-induced putative kinase protein 1 |
Gene Symbol | PINK1 |
Entrez Gene | 65018(Human) |
SwissProt | Q9BXM7(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of PINK1 expression in A431 (A), mouse brain (B) whole cell lysates. (Predicted band size: 62 kD; Observed band size: 62 kD)
Immunohistochemical analysis of PINK1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of PINK1 staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 555 was used to stain Actin filaments (red).
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