PD-L1 Rabbit Monoclonal Antibody(ARA730)
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
Product Details
WB | 1:2500-1:5000 |
IHC | 1:100-1:200 |
IF/ICC | 1:2000-1:10000 |
FC | 1:50-1:200 |
IP | 1:30 |
Description | Rabbit Monoclonal Antibody to PD-L1 |
Antibody Type | Primary antibody |
Predicted MW | 33kDa |
Immunogen | Recombinant full length protein corresponding to Human PD-L1. The immunogen contains the specific extracellular domain of huPD-L1 (F19-T239). |
Purification | ProA affinity purified IgG |
Form/Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. |
Alternative Names | B7H1; PDCD1L1; PDCD1LG1; PDL1; Programmed cell death 1 ligand 1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7 homolog 1; B7-H1; CD274 |
Gene Symbol | CD274 |
Entrez Gene | 29126(Human) |
Swissprot | Q9NZQ7 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
All lanes: Anti-PD-L1 antibody at 1:5,000 dilution
Predicted MW: 33 kDa
Observed MW: 40-50 kDa
Lane 1: HEK293 Overexpression of HuPD-L1
Lane 2: HEK293
Lysates at 5 μg per lane
2nd Ab:
GAR HRP(H+L) 1:10,000
Exposure: 100s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung adenocarcinoma tissue labelling PD-L1 with ARA730 at 1:200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
ARA730 staining 293 cells trasnfected with PD-L1 gene by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:10,000) at 4°C. An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (1:500).
Overlay histogram showing 293 cells transfected with PD-L1 gene stained with ARA730. The cells were then incubated in the antibody (ARA730, 1:200 dilution) in 1x PBS/1% BSA for 30 min at at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
PD-L1 was immunoprecipitated from 0.2mg of 293 whole cells lysate transfected with PD-L1 gene with ARA730 at 1:30 dilution.<br>
2nd Ab: GAR HRP for IP 1:500<br>
Lane 1: ARA730 IP in 293 whole cell lysate transfected with PD-L1 gene<br>
Lane 2: PBS instead of ARA730 in 293 whole cell lysate transfected with PD-L1 gene<br>
Lane 3: 293 whole cell lysate transfected with PD-L1 gene, 2 μg (input)<br>
Exposure: 10s
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