PCB Rabbit Monoclonal Antibody(ARA718)

Datasheet

MSDS

Key features and details

Target: PCB
Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Application: WB, IHC, IF/ICC, FC, IP
Storage: -20°C

Brand:

CAT.NO. : ARA6493

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Pyruvate carboxylase (PC) encoded by the gene PC is an enzyme (EC 6.4.1.1) of the ligase class that catalyzes (depending on the species) the physiologically irreversible carboxylation of pyruvate to form oxaloacetate (OAA). It is an important anaplerotic reaction that creates oxaloacetate from pyruvate. PC contains a biotin prosthetic group and is typically localized to the mitochondria in eukaryotes with exceptions to some fungal species such as Aspergillus nidulans which have a cytosolic PC. PC requires magnesium and zinc or manganese for catalysis. PC from different organisms exhibit varying degrees of activation by acetyl-CoA, but vertebrate PC typically requires it for activity.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:1,000 - 1:5,000
IHC	1:180 - 1:200
IF/ICC	1:200 - 1:250
FC	1:800 - 1:1,000
IP	1-2μg/sample

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Recombinant protein within Human PCB aa 450-1178.
Molecular Weight	Predicted band size: 130 kDa
Positive Control	HepG2 cell lysate, MCF7 cell lysate, A549 cell lysate, Huh7 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse kidney tissue lysate, Mouse liver tissue lysate, Mouse lymph node tissue lysate, Rat liver tissue lysate, Rat brain tissue lysate, HepG2, PC-12, mouse liver tissue, mouse lymph node tissue, rat liver tissue, rat brain tissue.
Conjugation	unconjugated
Form/Buffer	PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype	IgG
Purification	Protein A affinity purified.

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of PCB on different lysates with PCB Rabbit Monoclonal Antibody(ARA718).

Immunocytochemistry analysis of HepG2 cells labeling PCB with PCB Rabbit Monoclonal Antibody(ARA718).

Immunohistochemical analysis of paraffin-embedded rat liver tissue with PCB Rabbit Monoclonal Antibody(ARA718).

Flow cytometric analysis of PC-12 cells labeling PCB.

PCB was immunoprecipitated in 0.2mg HeLa cell lysate with PCB Rabbit Monoclonal Antibody(ARA718) at 2 µg/10 µl beads.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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