Nucleostemin Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
Product Details
WB | 1:500 - 1:2000 |
IF/ICC | 1:10 - 1:100 |
Description | Rabbit polyclonal antibody to Nucleostemin |
Specificity | Recognizes endogenous levels of Nucleostemin protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human Nucleostemin |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 60; Observed: 62 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | E2IG3; NS; Guanine nucleotide-binding protein-like 3; E2-induced gene 3 protein; Novel nucleolar protein 47; NNP47; Nucleolar GTP-binding protein 3; Nucleostemin |
Gene Symbol | GNL3 |
Entrez Gene | 26354(Human) |
SwissProt | Q9BVP2(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of Nucleostemin expression in HEK293T (A) whole cell lysates. (Predicted band size: 60; 61 kD; Observed band size: 62 kD)
Immunofluorescent analysis of Nucleostemin staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
FAQs
New Products
