NEFH Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:200 |
IP | 1:10 - 1:50 |
Description | Rabbit polyclonal antibody to NEFH |
Specificity | Recognizes endogenous levels of NEFH protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human NEFH |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 105; Observed: 150; 240 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | KIAA0845; NFH; Neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; Neurofilament triplet H protein |
Gene Symbol | NEFH |
Entrez Gene | 4744(Human); 380684(Mouse) |
SwissProt | P12036(Human); P19246(Mouse); P16884(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of NEFH expression in mouse brain (A) whole cell lysates. (Predicted band size: 105; 112 kD; Observed band size: 150; 240 kD)
Immunofluorescent analysis of NEFH staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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