MYC tag Rabbit Monoclonal antibody(ARA678)

Datasheet

MSDS

MYC tag Rabbit Monoclonal antibody(ARA678)

Key features and details

Target: MYC tag
Host: Rabbit
Reactivity: Species independent
Clonality: Monoclonal
Application: WB, IF/ICC, FC, IP, ELISA
Storage: -20°C

Brand:

CAT.NO. : ARA6439

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:5000 - 1:40000
FC	0.50 ug per 10^6 cells in a 100 µl suspension
IF/ICC	1:500 - 1:3000
IP	0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Immunogen	Peptide
Isotype	Mouse / IgG1
Antibody Class	Monoclonal
Product Type	Antibody
Full Name	MYC tag
Alternative Names	myc, EQKLISEEDL, MYC-tag
Conjugate Type	Unconjugated
Form	Liquid
Purification	Protein G purification
UNIPROT ID	P01106
Form/Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot of Myc-tagged fusion protein with MYC tag Rabbit Monoclonal antibody(ARA678) at various dilutions.

Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using MYC tag Rabbit Monoclonal antibody(ARA678) at dilution of 1:1000 and Fluor 488-Conjugated AffiniPure Mouse IgG(H+L) Goat Antibody.

IP result of MYC tag Rabbit Monoclonal antibody(ARA678) (IP:4ug; Detection:1:10000) with Transfected HEK-293 cells lysate 300ug.

1x10^6 HEK-293 transfected with GFP-MYC were intracellularly stained with 0.25 ug MYC tag Rabbit Monoclonal antibody(ARA678) and Fluor 647 Mouse IgG(H+L) Goat Antibody . Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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