Mouse IL-13 ARExSet® antibody pair for ELISA
Protocol Booklet 
Key features and details
- Species:
- Size:
- Sample Volume:
- Standard Curve Range:
- Assay type:
- Sensitivity:
- Operation Duration:
- Assay type:
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Brand:
CAT.NO. : ASEM0007
US$ Please choose
US$ Please choose
Product Details
Background
IL-13 (Interleukin-13) plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. It promotes B cell activation, immunoglobulin class switching to IgE, and the upregulation of CD23/Fc epsilon RII. IL-13 signals through a receptor complex containing IL-13 R alpha 1 and IL-4 R alpha. This complex also functions as the type 2 IL-4 receptor. IL-13 R alpha 2 binds IL-13 and prevents IL-13 from signaling through the IL-13 R alpha 1/IL-4 R alpha complex. IL-13-bound IL-13 R alpha 2 can directly promote tumor cell invasiveness and the development of tissue fibrosis.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0304 | 0.0325 | 0.0315 | |
| 7.81 | 0.1042 | 0.1016 | 0.1029 | 0.0715 |
| 15.63 | 0.1765 | 0.1744 | 0.1755 | 0.1440 |
| 31.25 | 0.3083 | 0.2784 | 0.2934 | 0.2619 |
| 62.50 | 0.5267 | 0.4747 | 0.5007 | 0.4693 |
| 125.00 | 0.9530 | 0.9234 | 0.9382 | 0.9068 |
| 250.00 | 1.6390 | 1.5760 | 1.6075 | 1.5761 |
| 500.00 | 2.7360 | 2.7190 | 2.7275 | 2.6961 |
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
ARExSet® Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.
- 96 well microplates: AREX Biosciences, Catalog # DSEP01. Plate Sealers: AREX Biosciences, Catalog # DSSF01.
- Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm fi ltered . AREX Biosciences, Catalog # DSCB01.
- Blocking Buffer: AREX Biosciences, Catalog # DSBB01.
- Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.
- Assay Buffer: 0.5%BSA，0.05%Tween20，PBS Solution.AREX Biosciences, Catalog # DSAB01
- Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01.
- Stop Solution: 0.5mol/ml H2SO4. AREX Biosciences, Catalog # DSSS01.
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