Mouse CD68/SR-D1 ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEM0042
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Size:
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Product Details
Background
CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein that belongs to the LAMP family of molecules. CD68 is expressed on monocytes and macrophages and serves as a scavenger receptor for oxidized LDL.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0409 |
0.0415 |
0.0412 |
|
|
78.13 |
0.1598 |
0.1527 |
0.1563 |
0.1151 |
|
156.25 |
0.2903 |
0.2588 |
0.2746 |
0.2334 |
|
312.50 |
0.5202 |
0.4834 |
0.5018 |
0.4606 |
|
625.00 |
0.9210 |
0.8673 |
0.8942 |
0.8530 |
|
1250.00 |
1.6190 |
1.6390 |
1.6290 |
1.5878 |
|
2500.00 |
2.9040 |
2.9550 |
2.9295 |
2.8883 |
|
5000.00 |
4.2213 |
4.1789 |
4.2001 |
4.1589 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(pg/ml) |
141.4 |
654.7 |
1898.7 |
129.9 |
593.6 |
1759.2 |
|
Standard Deviation |
5.9 |
35.1 |
107.5 |
7.6 |
34.7 |
80.1 |
|
Coefficient of Variation(%) |
4.2 |
5.4 |
5.7 |
5.8 |
5.8 |
4.6 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of mouse CD68/SR-D1 into health mouse serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 119% with an overall mean recovery of 95%.
The recovery ranged from 80% to 119% with an overall mean recovery of 95%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1438.48-2557.23 | 100 | 1971.16 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of IL-1 beta in this assay. No medical histories were available for the donors. n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
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