Mouse alpha 1 antitrypsin ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEMY0052
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Product Details
Background
Serpin A1 is the archetypal member of the Serpin superfamily of the serine protease inhibitors. As one of the most abundant proteinase inhibitors in the circulation, it is synthesized in the liver and secreted into the bloodstream with the major function to protect tissues against neutrophil elastase. A severe serpin A1 deficiency leads to several clinical complications such as pulmonary emphysema, juvenile hepatitis, cirrhosis, and hepatocellular carcinoma. The deficiency is caused by point mutations in naturally occurring serpin A1 variants (over 70 are known). For example, the Z variant (Glu342 to Lys) forms intracellular inclusion bodies, is not secreted, and leads to a severe serpin A1 deficiency.
Typical data
|
ng/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0085 |
0.0087 |
0.0086 |
|
|
13.72 |
0.0168 |
0.0182 |
0.0175 |
0.0089 |
|
41.15 |
0.0345 |
0.0348 |
0.0347 |
0.0261 |
|
123.46 |
0.0903 |
0.0873 |
0.0888 |
0.0802 |
|
370.37 |
0.2459 |
0.2427 |
0.2443 |
0.2357 |
|
1111.11 |
0.6791 |
0.6760 |
0.6776 |
0.6690 |
|
3333.33 |
1.7800 |
1.7870 |
1.7835 |
1.7749 |
|
10000.00 |
3.7750 |
3.8150 |
3.7950 |
3.7864 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(ng/ml) |
203.5 |
1135.4 |
4091.5 |
165.0 |
911.8 |
3156.6 |
|
Standard Deviation |
15.0 |
70.0 |
273.9 |
8.7 |
57.5 |
117.3 |
|
Coefficient of Variation(%) |
7.4 |
6.2 |
6.7 |
5.3 |
6.3 |
3.7 |
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mouse alpha 1 antitrypsin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 120% with an overall mean recovery of 102%.
The recovery ranged from 90% to 120% with an overall mean recovery of 102%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 210.39-335.68 | 100 | 288.48 |
Serum/Plasma – Thirty samples from apparently healthy mice were evaluated for the presence of alpha 1 antitrypsin in this assay. No medical histories were available for the donors.
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