MCM3 Mouse Monoclonal Antibody(C3742)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:2000 |
FC | 1:10 - 1:50 |
Description | Mouse monoclonal antibody to MCM3 |
Specificity | Recognizes endogenous levels of MCM3 protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human MCM3. The exact sequence is proprietary. |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 90 kD; Observed: 100 kD |
Form/Buffer | Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | DNA replication licensing factor MCM3; DNA polymerase alpha holoenzyme-associated protein P1; P1-MCM3; RLF subunit beta; p102 |
Gene Symbol | MCM3 |
Entrez Gene | 4172(Human); 17215(Mouse) |
SwissProt | P25205(Human); P25206(Mouse) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of MCM3 expression in CHO (A), NIH3T3 (B), Hela (C), HepG2 (D), C2C12 (E), PC12 (F) whole cell lysates. (Predicted band size: 90 kD; Observed band size: 100 kD)
Flow cytometric analysis of Hela cells using Anti-MCM3 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AREX® Fluor 488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
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