Mammal Insulin ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 60.07 pg/ml (50 μl) ；314.56 pg/ml (10 μl)
Standard Curve Range: 312.5~20000 pg/ml
Standard Curve Gradient: 7 Points
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0038

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Size:

96T

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Product Details

Background

Insulin and the IGFs are members of the insulin family of molecules. IGF-I and II are structurally homologous to proinsulin, and can be thought of as taking the shape of an exaggerated, inverted letter G. Intrachain disulfide bonds link sections of the G. The single chain insulin propeptide consists of a 30 amino acid B chain (aa 2554), a C-peptide (aa 5589), and a 21 aa A chain (aa 90110). Removal of the C-peptide by proteolysis enables the formation of mature Insulin, a disulfidelinked heterodimer of the A and B chains. Circulating C-peptide levels are elevated in hyperinsulinism, obesity, and type II diabetes.

Typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0105	0.0105	0.0105
312.50	0.0206	0.0199	0.0203	0.0098
625.00	0.0550	0.0538	0.0544	0.0439
1250.00	0.2002	0.1978	0.1990	0.1885
2500.00	0.8078	0.8195	0.8137	0.8032
5000.00	2.2280	2.1400	2.1840	2.1735
10000.00	3.6520	3.7690	3.7105	3.7000
20000.00	4.2653	4.2940	4.2797	4.2692

Precision

Item	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
	22	22	22	6	6	6
Average(pg/ml)	581.0	2316.1	7446.9	579.8	2277.0	7736.2
Standard Deviation	12.8	34.2	243.7	14.4	34.5	230.5
Coefficient of Variation(%)	2.2	1.5	3.3	2.5	1.5	3.0

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of mammal Insulin into health serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 120% with an overall mean recovery of 110%.

Sample Values

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	132.03-2758.63	100	1027.08

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Insulin in this assay. No medical histories were available for the donors.

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