Mammal GFAP ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0178
US$ Please choose
US$ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
Product Details
Background
GFAP (Glial fibrillary acidic protein) is a type III intermediate filament protein. It is the major component of astrocyte intermediate filament. Defects in GFAP are a cause of Alexander disease. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. At the amino acid sequence level, human GFAP shares 91% and 90% identity with rat and mouse GFAP, respectively.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0335 |
0.0361 |
0.0348 |
|
|
68.59 |
0.0570 |
0.0603 |
0.0587 |
0.0239 |
|
205.76 |
0.0843 |
0.0939 |
0.0891 |
0.0543 |
|
617.28 |
0.1807 |
0.1806 |
0.1807 |
0.1459 |
|
1851.85 |
0.4176 |
0.4205 |
0.4191 |
0.3843 |
|
5555.56 |
1.1570 |
1.1750 |
1.1660 |
1.1312 |
|
16666.67 |
2.8440 |
2.9960 |
2.9200 |
2.8852 |
|
50000.00 |
4.3945 |
4.5339 |
4.4642 |
4.4294 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(pg/ml) |
724.3 |
3800.8 |
14128.0 |
794.5 |
4527.5 |
16907.8 |
|
Standard Deviation |
2.2 |
17.4 |
76.9 |
6.7 |
25.3 |
78.4 |
|
Coefficient of Variation(%) |
4.7 |
6.5 |
5.2 |
3.8 |
5.4 |
5.8 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of Mammal GFAP into health serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 96% to 104% with an overall mean recovery of 101%.
The recovery ranged from 96% to 104% with an overall mean recovery of 101%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-23.29 | 75 | 5.29 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of GFAP in this assay. No medical histories were available for the donors.
New Products
