Lactoferrin Mouse Monoclonal Antibody(C3585)
WB | 1:500 - 1:4000 |
IF/ICC | 1:10 - 1:30 |
Description | Mouse monoclonal antibody to Lactoferrin |
Specificity | Recognizes endogenous levels of Lactoferrin protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human Lactoferrin. The exact sequence is proprietary. |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 78 kD; Observed: 69 kD |
Form/Buffer | Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | GIG12; LF; Lactotransferrin; Lactoferrin; Growth-inhibiting protein 12; Talalactoferrin |
Gene Symbol | LTF |
Entrez Gene | 4057(Human) |
SwissProt | P02788(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Western blot analysis of Lactoferrin expression in K562 (A) whole cell lysates. (Predicted band size: 78 kD; Observed band size: 69 kD)

Immunofluorescent analysis of Lactoferrin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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