L-Lactyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody(ARA873)-ChIP Grade

Datasheet

MSDS

L-Lactyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody(ARA873)-ChIP Grade

Key features and details

Target: L-Lactyl-Histone H3 (Lys18)
Host: Rabbit
Reactivity: Human, Mouse
Clonality: Monoclonal
Application: WB, ChIP, Cut&tag
Storage: -20°C

Brand:

CAT.NO. : ARA6699

US$ Please choose

Size:

50μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:500 - 1:1000
ChIP	6 μg/5×10⁶ cells
Cut&tag	1:100

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Isotype	IgG
Purification	Protein A
Cellular Localization	Nucleus
Form/Buffer	PBS, Glycerol, BSA
UniProt ID	P68431
Synonyms	H3K18la
Conjugate	Unconjugated
Specificity	Anti-L-Lactyl-Histone H3 (Lys18) Rabbit mAb (ChIP Grade) detects histone H3 only when it is L-lactylated at Lys18.
UniProt ID	P68431
Immunogen	L-lactylated human histone H3 (Lys18) peptide
MW (kDa)	15

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Peptide amount: 4 ng, 16 ng, 64 ng Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:2000 Primary Ab incubation: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 10 seconds The list of peptides used in the experiment is provided below. Lane 1: H3K18 L-lactyl. Lane 2: H3K18 acetyl. Lane 3: H3K18 crotonyl. Lane 4: H3K18 butyryl. Lane 5: H3K18 propionyl. Lane 6: H3K18 2-hydroxyisobutyryl. Lane 7: H3K18 β-hydroxybutyryl. Lane 8: H3K18 unmodified

Lysates: (-) HeLa cells; (+) HeLa cells treated with 25 mM sodium lactate for 24 hours Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 15 kDa Observed band size: 15 kDa

Lysates: (-) K562 cells; (+) K562 cells treated with 25 mM sodium lactate for 24 hours Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 15 kDa Observed band size: 15 kDa

Lysates: (-) NIH/3T3 cells; (+) NIH/3T3 cells treated with 25 mM sodium lactate for 24 hours Protein loading amount: 20 μg Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L pAb (HRP Conjugate) Exposure time: 60 seconds Predicted band size: 15 kDa Observed band size: 15 kDa

Sample: HeLa cells treated with 100 mM sodium lactate for 24 hours Cross-linking conditions: no cross-linking Amount of chromatin per IP: 5×106 cells Amount of Ab per IP: 6 μg Beads type and amount per IP: 50 μL of Protein A MagBeads Description: Chromatin immunoprecipitations were performed with 6 μg of normal rabbit IgG as a negative control. The immunoprecipitated DNA was quantified by real-time PCR using primers specific for the human GAPDH-CDS, LDHA promoter, LDHA, FOXO3a downstream, RAB20, and TUBBP10 regions. The data are presented as enrichment of each sample relative to the total amount of input at each amplicon.

Sample: HeLa cells treated with 100 mM sodium lactate for 24 hours Cell quantity: 1×105 cells Primary Ab dilution: 1:100 Primary Ab incubation: 4⁰C overnight Secondary Ab: Goat Anti-Rabbit IgG H&L pAb Secondary Ab dilution: 1:1400 Description: CUT&Tag was performed with Anti-L-Lactyl-Histone H3 (Lys18) Rabbit mAb alongside normal rabbit IgG as a negative control. The resulting DNA was sequenced on the Illumina NovaSeq platform with paired-end reads of 150 bp and a sequencing depth of 3 million reads. The figure shows significant H3K18la signal enrichments at the promoter regions of CD9, PLEKHG6, LTBR, and GAPDH.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

New Products

TRBC1 Mouse Monoclonal Antibody(ARA662)

Mouse monoclonal to TRBC1

OCT2 Mouse Monoclonal Antibody(C2325)

Mouse monoclonal antibody to OCT2

ARExVisual® DAB Buffer

Primary Antibody Diluent

ARExVisual® DAB chromogen (20×)

CD68 Rabbit Monoclonal Antibody(ARA633)