Kanamycin Residual ELISA Kit
Key features and details
- Limit of Detection (LOD):
- Spike Recovery Rate:
- Limit of Quantitation (LOQ):
- Repeatability (Intra-Batch CV):
- Detection Range:
- Measurement Deviation:
-
Brand:
CAT.NO. : ARK6617
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Size:
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Product Details
Background
Kanamycin, an aminoglycoside antibiotic, is used in the treatment of animal diseases, strain screening and the preparation of cell and gene therapy drug raw materials. Due to its neurotoxic and nephrotoxic properties, it can damage the 8th cranial nerve, leading to vestibular and cochlear injuries. Residues in animal-derived foods and biological drugs can affect human health and even cause allergic reactions. Regulatory authorities in Europe, the United States, and China require strict limits on its use.
AREX's Kanamycin ELISA Kit employs an indirect competitive ELISA method. The pre-coated kanamycin-conjugated antigen on the microplate strip competes with residual kanamycin in the sample for binding to the enzyme-labeled anti-kanamycin monoclonal antibody. After adding the enzyme-labeled secondary antibody and TMB substrate for color development, the absorbance is measured using a microplate reader. The absorbance is inversely proportional to the kanamycin content in the sample. This kit requires only approximately one hour for operation and has a linear range of 0.05 ng/mL–5 ng/mL.
AREX's Kanamycin ELISA Kit employs an indirect competitive ELISA method. The pre-coated kanamycin-conjugated antigen on the microplate strip competes with residual kanamycin in the sample for binding to the enzyme-labeled anti-kanamycin monoclonal antibody. After adding the enzyme-labeled secondary antibody and TMB substrate for color development, the absorbance is measured using a microplate reader. The absorbance is inversely proportional to the kanamycin content in the sample. This kit requires only approximately one hour for operation and has a linear range of 0.05 ng/mL–5 ng/mL.
Overview
|
Limit of Detection (LOD) |
≤ 0.05 ng/mL |
|
Spike Recovery Rate |
70%–130% |
|
Limit of Quantitation (LOQ) |
0.05 ng/mL |
|
Repeatability (Intra-Batch CV) |
≤15% |
|
Detection Range |
0.05–5 ng/mL |
|
Measurement Deviation |
≤15% |
Advantage
High Specificity: No cross-reactivity with penicillin, streptomycin sulfate, gentamicin or ampicillin.
High Sensitivity: Detection sensitivity as low as 0.05 ng/mL.
Convenient and Efficient: Two-step method, delivers results in approximately 1 hour.
Superior Performance: Excellent standard curve linearity, high accuracy and good repeatability.
High Sensitivity: Detection sensitivity as low as 0.05 ng/mL.
Convenient and Efficient: Two-step method, delivers results in approximately 1 hour.
Superior Performance: Excellent standard curve linearity, high accuracy and good repeatability.
Storage
The kit should be stored away from light at 2-8 ° C for 12 months. The unused kits should be kept away from light at 2-8 ° C after opening.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
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