Human Neuropilin-1/NRP1 ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEHY0175
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Product Details
Background
Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins. The amino acid sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively.
Typical data
|
ng/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0064 |
0.0061 |
0.0063 |
|
|
0.27 |
0.0235 |
0.0214 |
0.0225 |
0.0162 |
|
0.82 |
0.0624 |
0.0543 |
0.0584 |
0.0521 |
|
2.47 |
0.1677 |
0.1621 |
0.1649 |
0.1587 |
|
7.41 |
0.4859 |
0.4380 |
0.4620 |
0.4557 |
|
22.22 |
1.4320 |
1.3170 |
1.3745 |
1.3683 |
|
66.67 |
3.5090 |
3.4470 |
3.4780 |
3.4718 |
|
200.00 |
4.4662 |
4.5095 |
4.4879 |
4.4816 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(ng/ml) |
7.6 |
38.8 |
148.5 |
6.5 |
33.6 |
102.7 |
|
Standard Deviation |
0.3 |
1.8 |
2.7 |
0.4 |
2.0 |
2.5 |
|
Coefficient of Variation(%) |
3.6 |
4.5 |
1.8 |
6.0 |
6.0 |
2.5 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Neuropilin-1/NRP1 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 125% with an overall mean recovery of 97%.
The recovery ranged from 80% to 125% with an overall mean recovery of 97%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 407.50-604.49 | 100 | 514.05 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Neuropilin-1/NRP1 in this assay. No medical histories were available for the donors.
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