Human IL-2 Receptor alpha ELISA Kit

Key features and details

Specification: 96 Test
Sensitivity: 0.01 pg/ml (50 μl)；1.14 pg/ml (10 μl)；
Standard Curve Range: 1.37~1000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0170

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Size:

96T

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Product Details

Background

Two type1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha l or NR4, is now referred to as IL-13 R alpha 1. The high affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha l, is now referred to as IL-13 R alpha 2. Human IL-13 R alpha 2 was originally cloned from the Caki-1 human renal carcinoma cell line. The IL-13 R alpha 2 cDNA encodes a 380 amino acid (aa) residue precursor protein with a putative 26 aa residue signal peptide, a 317 residue extracellular domain, a 20 aa residue transmembrane region and a 17 aa residue cytoplasmic tail. Human and mouse IL‑13 R alpha 2 share 59% aa sequence identity. The extracellular domain of IL-13 R alpha 2 is also closely related to that of IL-13 R alpha 1. However, the 17 aa residue cytoplasmic domain of IL-13 R alpha 2 is much shorter than that of IL-13 R alpha 1, suggesting that the two receptors are functionally distinct. IL-13 R alpha 1 has been shown to combine with the IL-4 R to form a high-affinity receptor complex capable of transducing an IL‑13‑dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated. The amino-terminal 27 aa residues of the human and mouse IL‑13 R alpha 2 are nearly identical to that of a soluble mouse IL-13 binding protein purified from mouse serum and urine.

Typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0082	0.0086	0.0084
1.37	0.0274	0.0256	0.0265	0.0181
4.12	0.0649	0.0683	0.0666	0.0582
12.35	0.1922	0.1804	0.1863	0.1779
37.04	0.4898	0.4806	0.4852	0.4768
111.11	1.2600	1.1500	1.2050	1.1966
333.33	2.4250	2.4020	2.4135	2.4051
1000.00	3.6340	3.6390	3.6365	3.6281

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average(pg/ml)	19.2	115.7	350.8	18.1	103.6	361.9
Standard Deviation	0.9	5.4	18.3	0.7	4.9	14.8
Coefficient of Variation(%)	4.7	4.7	5.2	3.9	4.7	4.1

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-2 Receptor alpha into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 89% to 118% with an overall mean recovery of 106%.

Sample Values

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	722.90-1772.29	100	1340.94

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-2 Receptor alpha in this assay. No medical histories were available for the donors.

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