Human IL-12B p40 ARExSet® antibody pair for ELISA
Key features and details
- Species:
- Size:
- Sample Volume:
- Standard Curve Range:
- Assay type:
- Sensitivity:
- Operation Duration:
- Assay type:
-
Brand:
CAT.NO. : ASEH0098
US$ Please choose
US$ Please choose
Product Details
Background
Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0306 |
0.0305 |
0.0306 |
|
|
31.25 |
0.0919 |
0.0959 |
0.0939 |
0.0634 |
|
62.50 |
0.1711 |
0.1568 |
0.1640 |
0.1334 |
|
125.00 |
0.3216 |
0.2896 |
0.3056 |
0.2751 |
|
250.00 |
0.5620 |
0.5547 |
0.5584 |
0.5278 |
|
500.00 |
1.1000 |
0.9734 |
1.0367 |
1.0062 |
|
1000.00 |
1.8700 |
1.8180 |
1.8440 |
1.8135 |
|
2000.00 |
3.2590 |
3.1890 |
3.2240 |
3.1935 |
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
ARExSet® Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.
- 96 well microplates: AREX Biosciences, Catalog # DSEP01. Plate Sealers: AREX Biosciences, Catalog # DSSF01.
- Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm fi
ltered . AREX Biosciences, Catalog # DSCB01. - Blocking Buffer: AREX Biosciences, Catalog # DSBB01.
- Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.
- Assay Buffer: 0.5%BSA，0.05%Tween20，PBS Solution. AREX Biosciences, Catalog # DSAB01
Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01. - Stop Solution: 0.5mol/ml H2SO4. AREX Biosciences, Catalog # DSSS01.
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