Human Granzyme A/GZMA ARExSet® antibody pair for ELISA

Protocol Booklet

Human Granzyme A/GZMA ARExSet® antibody pair for ELISA

Key features and details

Species: Human
Size: 5 plates,15 plates,50 plates
Sample Volume: 50 μl
Standard Curve Range: 31.25 pg/ml-2000 pg/ml
Assay type: Not Ready-to-Use
Sensitivity: 0.08 pg/ml(50 μl)
Operation Duration: 120min
Assay type: Sandwich Elisa

Brand:

CAT.NO. : ASEH0129

US$ Please choose

Size:

5 plates 15 plates 50 plates

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.

Typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0126	0.0124	0.0125
31.25	0.0940	0.0933	0.0937	0.0812
62.50	0.1865	0.1921	0.1893	0.1768
125.00	0.3146	0.3234	0.3190	0.3065
250.00	0.5960	0.6138	0.6049	0.5924
500.00	1.1820	1.2050	1.1935	1.1810
1000.00	2.3567	2.2059	2.2813	2.2688
2000.00	4.0887	4.0571	4.0729	4.0604

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

ARExSet^®Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.

96 well microplates: AREX Biosciences, Catalog # DSEP01.

Plate Sealers: AREX Biosciences, Catalog # DSSF01.

Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2-7.4, 0.2 μm filtered . AREX Biosciences, Catalog # DSCB01.

Blocking Buffer: AREX Biosciences, Catalog # DSBB01.

Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.

Assay Buffer: 0.5%BSA, 0.05%Tween20, PBS Solution.AREX Biosciences, Catalog # DSAB01

Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01.

Stop Solution: 0.5 mol/ml H₂SO₄. AREX Biosciences, Catalog # DSSS01.

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