Human CXCL10/IP-10 ARExSet® antibody pair for ELISA

Key features and details

  • Species: Human
  • Size: 5 plates,15 plates,50 plates
  • Sample Volume: 50 μl
  • Standard Curve Range: 7.81 pg/ml -500 pg/ml
  • Assay type: Not Ready-to-Use
  • Sensitivity: 0.008 pg/ml(50 μl)
  • Operation Duration: 120min
  • Assay type: Sandwich Elisa
  • Brand:
CAT.NO. : ASEH0026
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Product Details
Background
IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily.
Typical data
pg/ml O.D. Average Corrected
0.00 0.0652 0.0647 0.0650
7.81 0.4118 0.4172 0.4145 0.3496
15.63 0.7663 0.8037 0.7850 0.7201
31.25 1.3670 1.3880 1.3775 1.3126
62.50 2.3370 2.4290 2.3830 2.3181
125.00 3.5480 3.6130 3.5805 3.5156
250.00 4.5404 4.4945 4.5175 4.4525
500.00 4.4632 4.7721 4.6177 4.5527
Product Features
  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
Kit Content
  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required

ARExSet®Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.

96 well microplates: AREX Biosciences, Catalog # DSEP01. 

Plate Sealers: AREX Biosciences, Catalog # DSSF01.

Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm filtered . AREX Biosciences, Catalog # DSCB01.

Blocking Buffer: AREX Biosciences, Catalog # DSBB01.

Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.

Assay Buffer: 0.5%BSA, 0.05%Tween20, PBS Solution.AREX Biosciences, Catalog # DSAB01

Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01.

Stop Solution: 0.5 mol/ml H2SO4. AREX Biosciences, Catalog # DSSS01.

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