Human CD62E ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0198
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Size:
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Product Details
Background
E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0179 |
0.0184 |
0.0182 |
|
|
8.23 |
0.0352 |
0.0343 |
0.0348 |
0.0166 |
|
24.69 |
0.0677 |
0.0684 |
0.0681 |
0.0499 |
|
74.07 |
0.1695 |
0.1696 |
0.1696 |
0.1514 |
|
222.22 |
0.4737 |
0.4691 |
0.4714 |
0.4533 |
|
666.67 |
1.2900 |
1.3210 |
1.3055 |
1.2874 |
|
2000.00 |
3.1610 |
3.2220 |
3.1915 |
3.1734 |
|
6000.00 |
4.4674 |
4.4727 |
4.4701 |
4.4519 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(pg/ml) |
163.0 |
726.7 |
2062.0 |
124.4 |
594.9 |
1727.9 |
|
Standard Deviation |
9.2 |
29.1 |
128.8 |
5.2 |
31.9 |
74.36 |
|
Coefficient of Variation(%) |
5.6 |
4.0 |
6.2 |
4.2 |
5.4 |
4.3 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CD62E into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 115% with an overall mean recovery of 105%.
The recovery ranged from 85% to 115% with an overall mean recovery of 105%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 8.16-43.12 | 100 | 19.69 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of CD62E in this assay. No medical histories were available for the donors.
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