Human CD27/TNFRSF7 ELISA Kit
Protocol Booklet 
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CAT.NO. : AEH0114
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Product Details
Background
Human CD27 is a lymphocyte-specific member of the TNF receptor superfamily. CD27 is expressed on a subset of human thymocytes and on the majority of mature T cells. CD27 expression is up-regulated after TCR stimulation. Within the CD4+ compartment, it is preferentially expressed on CD45RA+ cells. In contrast, it is preferentially expressed on CD45RO+ cells in the CD8+ compartment. CD27 also appeaars to be a potential marker for memory B cells. It exists as both a disulfide-linked dimer on the cell surface and as a soluble protein found in serum. Human CD27 is a 260 amino acid (aa) protein with a 20 aa signal, a 173 aa extracellular domain, a 20 aa transmembrane domain, and a 47 aa cytoplasmic domain. The ligand for CD27 is CD70. CD70 is expressed on thymic stromal cells and a small subset of activated T cells. Additionally a subset of activated B cells express CD70. The CD27/CD70 interaction appears to be a weak costimulatory pathway involved in T cell and B cell immune response. CD27/CD70 interactions may be more involved in controlling the expansion phase of an immune response. This would be in contrast to B7/CD28 interactions, which are important for the activation phase of immune responses.
typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0211 | 0.0219 | 0.0215 | |
| 13.72 | 0.0346 | 0.0309 | 0.0328 | 0.0113 |
| 41.15 | 0.0708 | 0.0662 | 0.0685 | 0.0470 |
| 123.46 | 0.1491 | 0.1387 | 0.1439 | 0.1224 |
| 370.37 | 0.3997 | 0.3502 | 0.3750 | 0.3535 |
| 1111.11 | 1.0700 | 1.1050 | 1.0875 | 1.0660 |
| 3333.33 | 2.7800 | 2.7570 | 2.7685 | 2.7470 |
| 10000.00 | 4.4793 | 4.4118 | 4.4456 | 4.4241 |
precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 235.3 | 1382.0 | 4675.0 | 207.7 | 1101.3 | 3437.3 |
| Standard Deviation | 16.2 | 90.3 | 227.3 | 10.3 | 68.9 | 259.5 |
| Coefficient of Variation(%) | 6.9 | 6.5 | 4.9 | 5.0 | 6.3 | 7.5 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
spike recovery
The spike recovery was evaluated by spiking 3 levels of human CD27/TNFRSF7 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 118% with an overall mean recovery of 103%.
The recovery ranged from 80% to 118% with an overall mean recovery of 103%.
sample values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | 114.75-12084.57 | 100 | 6777.71 |
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