Human BCMA/TNFRSF17 ARExSet® antibody pair for ELISA

Protocol Booklet

Human BCMA/TNFRSF17 ARExSet® antibody pair for ELISA

Key features and details

Species: Human
Size: 5 plates,15 plates,50 plates
Sample Volume: 50 μl
Standard Curve Range: 31.25 pg/ml -2000 pg/ml
Assay type: Not Ready-to-Use
Sensitivity: 7.08pg/ml(50 μl)
Operation Duration: 120min
Assay type: Sandwich Elisa

Brand:

CAT.NO. : ASEH0143

US$ Please choose

Size:

5 plates 15 plates 50 plates

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0188	0.0220	0.0204
31.25	0.0521	0.0514	0.0518	0.0314
62.50	0.0868	0.0816	0.0842	0.0638
125.00	0.1548	0.1610	0.1579	0.1375
250.00	0.4083	0.4151	0.4117	0.3913
500.00	1.0230	0.9800	1.0015	0.9811
1000.00	2.0360	2.0650	2.0505	2.0301
2000.00	3.6405	3.6040	3.6223	3.6019

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

ARExSet^®Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.

96 well microplates: AREX Biosciences, Catalog # DSEP01.

Plate Sealers: AREX Biosciences, Catalog # DSSF01.

Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2-7.4, 0.2 μm filtered . AREX Biosciences, Catalog # DSCB01.

Blocking Buffer: AREX Biosciences, Catalog # DSBB01.

Wash Buffer: 0.05% Tween 20 in PBS5, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.

Assay Buffer: 0.5%BSA, 0.05%Tween20, PBS Solution.AREX Biosciences, Catalog # DSAB01

Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01.

Stop Solution: 0.5 mol/ml H₂SO₄. AREX Biosciences, Catalog # DSSS01.

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