Human B7-H6/NCR3LG1 ELISA Kit
CAT.NO. : AEH0116
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Product Details
Background
B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins. Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif. Both of the Ig-like domains carry N-linked glycosylation. Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53% - 56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells. It does not show binding to NKp44, NKp46, or NKG2D. Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis. B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors. The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis.
typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0203 | 0.0208 | 0.0206 | |
| 20.48 | 0.0313 | 0.0305 | 0.0309 | 0.0104 |
| 51.20 | 0.0403 | 0.0421 | 0.0412 | 0.0207 |
| 128.00 | 0.0750 | 0.0767 | 0.0759 | 0.0553 |
| 320.00 | 0.1884 | 0.2017 | 0.1951 | 0.1745 |
| 800.00 | 0.6884 | 0.7111 | 0.6998 | 0.6792 |
| 2000.00 | 2.0969 | 2.2789 | 2.1879 | 2.1674 |
| 5000.00 | 4.0442 | 4.1880 | 4.1161 | 4.0956 |
precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 111.1 | 559.2 | 1692.1 | 103.7 | 522.2 | 1594.1 |
| Standard Deviation | 6.7 | 35.9 | 40.2 | 7.8 | 25.2 | 62.4 |
| Coefficient of Variation(%) | 6.0 | 6.4 | 2.4 | 7.5 | 4.8 | 3.9 |
spike recovery
The spike recovery was evaluated by spiking 3 levels of human B7-H6 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 102% to 119% with an overall mean recovery of 108%.
The recovery ranged from 102% to 119% with an overall mean recovery of 108%.
sample values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | 159.96-719.34 | 100 | 459.11 |
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