HSP27 Mouse Monoclonal Antibody(C3352)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:100 |
Description | Mouse monoclonal antibody to HSP27 |
Specificity | Recognizes endogenous levels of HSP27 protein. |
Antibody Type | Primary antibody |
Imnunogen | Purified recombinant human Hsp27 protein fragments expressed in E.coli. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 23 kD; Observed: 27 kD |
Form/Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
Alternative Names | HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27 |
Gene Symbol | HSPB1 |
Entrez Gene | 3315(Human) |
SwissProt | P04792(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of HSP27 expression in Hela (A), Hela (B), A431 (C), COS7 (D), Lncap (E), MCF7 (F) whole cell lysates. (Predicted band size: 23 kD; Observed band size: 27 kD)
Immunofluorescent analysis of HSP27 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.
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