His-tag Mouse Monoclonal Antibody(C784)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:2000 - 1:5000 |
IF/ICC | 1:200 - 1:500 |
IP | 1:100 - 1:200 |
Description | Recombinant mouse monoclonal antibody to His-tag |
Specificity | Recognizes C-terminal, internal, and N-terminal His-tag fusion proteins. |
Antibody Type | Primary antibody, Recombinant, Tag |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence of His-tag. The exact sequence is proprietary. |
Purification | This antibody is purified by protein A affinity chromatography. |
Molecular Weight | N/A |
Form/Buffer | Liquid in PBS, pH 7.4, containing 50% glycerol, 0.05% BSA and 0.01% sodium azide. |
Alternative Names | |
Gene Symbol | |
Entrez Gene | |
SwissProt |
Western blot analysis of His-tag in 293F transfected with a vector overexpressing His tag (A), 293F transfected with an empty vector (B).
Immunofluorescent analysis of His-tag staining in 293T cells transfected with a His-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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